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  • Open Access

    ARTICLE

    Reprogramming the Metabolome of Centella asiatica (L.) Urban Callus: Profiling of Newly Synthesized Cryptic Anthocyanins Triggered by LED Light Exposure

    Claude Y. Hamany Djande, Paul A. Steenkamp, Ian A. Dubery*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1269-1286, 2025, DOI:10.32604/phyton.2025.062392 - 30 April 2025

    Abstract Centella asiatica L., a medicinal herb, has attracted substantial interest in research as well as commercial domains due to its bioactive compounds which include the pentacyclic triterpenoid centellosides, and in addition, hydroxy. In addition, hydroxycinnamic acid conjugates as well as flavonoids. The latter is the major class of secondary plant metabolites and comprises various subclasses, including anthocyanidins. Anthocyanins are rarely reported in extracts from C. asiatica and differ structurally due to a flavylium (2-phenylchromenylium) ion that carries a positive charge at the oxygen atom of the C-ring of the basic flavonoid structure. Callus of C. asiatica was initiated… More >

  • Open Access

    ARTICLE

    Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period

    Kesu Wei1, Guangju Liu2, Bin Wei3, Qifang Zhang4, Shengjiang Wu1, Zhaowei Li2,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1287-1298, 2025, DOI:10.32604/phyton.2025.062385 - 30 April 2025

    Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

  • Open Access

    ARTICLE

    Drought-Ready Soybean Resilience: Molecular Marker-Based Screening and Selection of Superior Genotypes

    Muhammad Imran Khan1, Fahad Masoud Wattoo1, Kamran Shehzad Bajwa2,*, Zahid Akram1, Sabahat Noor2, Zaheer Abbas2, Rashid Mehmood Rana1, Muhammad Tahir Akram3, Shaukat Ali2, Rai Muhammad Amir4, Ghulam Muhammad Ali5

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1299-1318, 2025, DOI:10.32604/phyton.2025.062323 - 30 April 2025

    Abstract The agricultural sector is notably affected by climate change, especially soybeans, which may face diminished yields because of severe water shortages. The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement. This study employed 10 simple sequence repeat (SSR) markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23. The results of the screening experiment (PEG-6000) revealed that the soybean genotypes SPS13, SPS195, PGRB83, and 39982 exhibited significant correlations in growth parameters. The results of molecular characterization indicated that five out of ten molecular… More >

  • Open Access

    ARTICLE

    Enhancing Salt Stress Tolerance in Portulaca oleracea L. Using Ascophyllum nodosum Biostimulant

    Zahra Alhawsa*, Rewaa Jalal, Nouf Asiri

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1319-1337, 2025, DOI:10.32604/phyton.2025.061918 - 30 April 2025

    Abstract Salinity stress is a major constraint on agricultural productivity, particularly in arid and semi-arid regions. This study evaluated the potential of Ascophyllum nodosum extract (ANE) in mitigating salinity-induced stress and enhancing the growth and physiological performance of Portulaca oleracea L. under NaCl concentrations of 0, 50, 70, and 100 mM for 50 days. A two-way ANOVA assessed the effects of NaCl concentration, ANE treatment, and their interaction. The results showed that ANE significantly increased plant height at 50 mM NaCl (p = 0.0011) but had no effect at higher salinity levels (p > 0.05). Shoot dry weight was… More >

  • Open Access

    ARTICLE

    Impact of Drought, Salinity, and Waterlogging on Wheat: Physiological, Biochemical Responses, and Yield Implications

    Mudasser Mehmood1,*, Zoahaib Aslam Khan1, Adil Mehmood2, Madiha Zaynab3, Muhammad Atiq ur Rahman4, Mohammad Khalid Al-Sadoon5, M. Harshini6, Ling Shing Wong7

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1111-1135, 2025, DOI:10.32604/phyton.2025.059812 - 30 April 2025

    Abstract Wheat (Triticum aestivum L.) is a staple crop critical for global food security, yet its productivity is significantly affected by abiotic stresses such as drought, salinity, and waterlogging, which are exacerbated by climate change. This study evaluated the effects of these stresses on vegetative growth, physiological responses, and yield. Field experiments were conducted using a Randomized Complete Block Design (RCBD) at the Mona Reclamation Experimental Project (MREP), WAPDA, Bhalwal, Sargodha, Punjab Pakistan. Stress treatments included three levels of drought (25%, 50%, and 75% field capacity), salinity (4, 8, and 12 dS/m), and waterlogging (24, 48, and… More >

  • Open Access

    EDITORIAL

    What Makes a Good Surgeon? What I Learned Watching Hardy Hendren

    Canadian Journal of Urology, Vol.32, No.2, pp. 71-72, 2025, DOI:10.32604/cju.2025.066690 - 30 April 2025

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    The cost and guideline adherence of direct-to-consumer telemedicine companies offering gender-affirming hormone therapy

    Nicholas Sellke1,2,*, Erin Jesse1,2, Justin M. Dubin3, Tomislav D. Medved1,2, Neha S. Basti4, Janvi Ramchandra2, Robert E. Brannigan4, Joshua A. Halpern4, Nannan Thirumavalavan1,2

    Canadian Journal of Urology, Vol.32, No.2, pp. 89-94, 2025, DOI:10.32604/cju.2025.065004 - 30 April 2025

    Abstract Introduction: Direct-to-consumer (DTC) telemedicine has emerged as an option for transgender patients seeking gender affirming hormone therapy (GAHT). We aimed to characterize the healthcare services provided by DTC telemedicine companies offering GAHT and to compare their costs to a tertiary care center. Methods: We identified DTC telemedicine platforms offering GAHT via internet searches and extracted information from their websites related to evaluation, treatment, monitoring, and cost. Cost of the DTC GAHT was compared to cost for comparable services at a tertiary care center. Results: Six DTC companies were identified. All platforms utilized an informed consent model… More >

  • Open Access

    ARTICLE

    The intersection of histologies: navigating the complexity of a renal collision tumor

    Tatiana Henriksson1,*, Katharina Mitchell2, Reima El Naili3, Ali Hajiran2

    Canadian Journal of Urology, Vol.32, No.2, pp. 95-99, 2025, DOI:10.32604/cju.2025.065002 - 30 April 2025

    Abstract Renal cell carcinoma is a heterogeneous group of renal tumors characterized by several histological subtypes. Herein, we discuss an unusual case of a 55-year-old male who presented as a consultation to our urology clinic with an incidentally found renal mass. After shared decision making patient proceeded with a Robotic Assisted Laparoscopy (RAL) left sided partial nephrectomy. Final pathology confirmed the presence of high nuclear grade mixed clear cell and papillary renal cell carcinoma (RCC) of the left kidney (pT3aN0M0). This case elucidates a very rare incidence of a patient seen to have a collision tumor, More >

  • Open Access

    LEGENDS IN UROLOGY

    Legends in Urology: Reflections on a Career in Academic Urology

    Peter T. Scardino

    Canadian Journal of Urology, Vol.32, No.2, pp. 73-80, 2025, DOI:10.32604/cju.2025.064714 - 30 April 2025

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Comparison of clinical effectiveness and safety of 272 µm and 365 µm holmium lasers in retrograde intrarenal surgery

    Demirhan Örsan Demir1,*, Yusuf Gökkurt1, Salih Bürlükkara2, Turgay Kaçan1, Ali Kaan Yıldız1, Ufuk Bozkurt2, Tolga Karakan1

    Canadian Journal of Urology, Vol.32, No.2, pp. 111-118, 2025, DOI:10.32604/cju.2025.063970 - 30 April 2025

    Abstract Introduction: Surgeons typically prefer 270 µm and 272 µm laser probes in retrograde intrarenal surgery (RIRS) due to the reduced deflection capacity of flexible ureterorenoscopes when using larger probe diameters. This study aims to investigate the effects of 272 and 365 µm holmium laser probes on operative time, clinical efficacy, and complication rates in RIRS. Materials and Methods: A total of 285 patients with proximal ureteral or kidney stones who met the inclusion criteria were enrolled in the study. Patients were divided into two groups based on laser probe thickness: 272 µm and 365 µm. Stone-free… More >

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